Part:BBa_K1467205:Design

AvrBS3 TAL Effector - Induces BS3

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1097
  Illegal NheI site found at 3050
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 3209
  Illegal BamHI site found at 4
  Illegal BamHI site found at 2817
  Illegal BamHI site found at 3412
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 770
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Domestication for GoldenGate by removal of internal BsaI and BpiI recognition sequences.

Codon-optimised for expression in dicotyledenous plants

Source

Xanthomonas vesicatoria